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recombinant pdgf d  (Proteintech)


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    Structured Review

    Proteintech recombinant pdgf d
    Recombinant Pdgf D, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdgf+d/PDGFD+Antibody/pm41758927-311-18-23
    Average 94 stars, based on 16 article reviews
    recombinant pdgf d - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Exploring the role of cytochrome P450 family 1 subfamily B member 1 and quercetin in modulating neuropathic pain after spinal cord injury
    Article Snippet: .. The slides were allowed to equilibrate at room temperature for 1 h. Subsequently, 0.5% Triton X-100 permeabilization solution (cat. no. 9036-19-5; Sigma-Aldrich; Merck KGaA) was applied to the sections and incubated at 4°C for 5 min. Ready-to-use goat serum blocking buffer (Beyotime Institute of Biotechnology) was then applied, followed by incubation at room temperature for 1 h. The sections were incubated overnight at 4°C with the following primary antibodies: CYP1B1 (1:100 dilution; cat. no. 18505-1-AP; Proteintech Group, Inc.) and PDGF-D (1:100 dilution; cat. no. 14075-1-AP; Proteintech Group, Inc.). .. Afterward, the sections were stained for 1 h with the following secondary antibodies: Goat anti-mouse IgG H&L (1:500; cat. no. ab150113; Abcam) and goat anti-rabbit IgG H&L (1:500; cat. no. ab150080; Abcam).

    Article Title: Exploring the role of cytochrome P450 family 1 subfamily B member 1 and quercetin in modulating neuropathic pain after spinal cord injury.
    Article Snippet: .. The slides were allowed to equilibrate at room temperature for 1 h. Subsequently, 0.5% Triton X‐100 permeabilization solution (cat. no. 9036‐19‐5; Sigma‐Aldrich; Merck KGaA) was applied to the sections and incubated at 4 ̊C for 5 min. Ready‐to‐use goat serum blocking buffer (Beyotime institute of Biotechnology) was then applied, followed by incubation at room temperature for 1 h. The sections were incubated overnight at 4 ̊C with the following primary antibodies: CYP1B1 (1:100 dilution; cat. no. 18505‐1‐AP; Proteintech Group, Inc.) and PDGF‐D (1:100 dilution; cat. no. 14075‐1‐AP; Proteintech Group, inc.). afterward, the sections were stained for 1 h with the following secondary antibodies: Goat anti‐mouse IgG H&L (1:500; cat. no. ab150113; Abcam) and goat anti‐rabbit IgG H&L (1:500; cat. no. ab150080; Abcam). .. Finally, cell nuclei in the tissue sections were labeled using 4',6‐diamidino‐2‐phe‐ nylindole (cat. no. F6057; Sigma‐Aldrich; Merck KGaA).

    Article Title: Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.
    Article Snippet: .. Then, the sections were incubated with monoclonal anti-PDGF-A, PDGF-B, PDGF-C (Santa Cruz Biotechnology, Inc, USA), and PDGF-D (Proteintech Group, Inc, USA) antibodies and antiPDGFR-a and PDGFR-b (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies produced in rabbit (1:50) for 18 h at 4 C. Subsequently, the sections were incubated with a secondary biotinylated anti-rabbit IgG antibody (Santa Cruz Biotechnology), diluted 1:200 in phosphate-buffered saline containing 1% normal goat serum for 45 min. .. The sections were incubated with avidin-biotin-HRP complex (1:600, Vectastain Elite ABC kits; Vector Laboratories, Burlingame, CA, USA) for 45 min.

    Blocking Assay:

    Article Title: Exploring the role of cytochrome P450 family 1 subfamily B member 1 and quercetin in modulating neuropathic pain after spinal cord injury
    Article Snippet: .. The slides were allowed to equilibrate at room temperature for 1 h. Subsequently, 0.5% Triton X-100 permeabilization solution (cat. no. 9036-19-5; Sigma-Aldrich; Merck KGaA) was applied to the sections and incubated at 4°C for 5 min. Ready-to-use goat serum blocking buffer (Beyotime Institute of Biotechnology) was then applied, followed by incubation at room temperature for 1 h. The sections were incubated overnight at 4°C with the following primary antibodies: CYP1B1 (1:100 dilution; cat. no. 18505-1-AP; Proteintech Group, Inc.) and PDGF-D (1:100 dilution; cat. no. 14075-1-AP; Proteintech Group, Inc.). .. Afterward, the sections were stained for 1 h with the following secondary antibodies: Goat anti-mouse IgG H&L (1:500; cat. no. ab150113; Abcam) and goat anti-rabbit IgG H&L (1:500; cat. no. ab150080; Abcam).

    Article Title: Exploring the role of cytochrome P450 family 1 subfamily B member 1 and quercetin in modulating neuropathic pain after spinal cord injury.
    Article Snippet: .. The slides were allowed to equilibrate at room temperature for 1 h. Subsequently, 0.5% Triton X‐100 permeabilization solution (cat. no. 9036‐19‐5; Sigma‐Aldrich; Merck KGaA) was applied to the sections and incubated at 4 ̊C for 5 min. Ready‐to‐use goat serum blocking buffer (Beyotime institute of Biotechnology) was then applied, followed by incubation at room temperature for 1 h. The sections were incubated overnight at 4 ̊C with the following primary antibodies: CYP1B1 (1:100 dilution; cat. no. 18505‐1‐AP; Proteintech Group, Inc.) and PDGF‐D (1:100 dilution; cat. no. 14075‐1‐AP; Proteintech Group, inc.). afterward, the sections were stained for 1 h with the following secondary antibodies: Goat anti‐mouse IgG H&L (1:500; cat. no. ab150113; Abcam) and goat anti‐rabbit IgG H&L (1:500; cat. no. ab150080; Abcam). .. Finally, cell nuclei in the tissue sections were labeled using 4',6‐diamidino‐2‐phe‐ nylindole (cat. no. F6057; Sigma‐Aldrich; Merck KGaA).

    Staining:

    Article Title: Exploring the role of cytochrome P450 family 1 subfamily B member 1 and quercetin in modulating neuropathic pain after spinal cord injury.
    Article Snippet: .. The slides were allowed to equilibrate at room temperature for 1 h. Subsequently, 0.5% Triton X‐100 permeabilization solution (cat. no. 9036‐19‐5; Sigma‐Aldrich; Merck KGaA) was applied to the sections and incubated at 4 ̊C for 5 min. Ready‐to‐use goat serum blocking buffer (Beyotime institute of Biotechnology) was then applied, followed by incubation at room temperature for 1 h. The sections were incubated overnight at 4 ̊C with the following primary antibodies: CYP1B1 (1:100 dilution; cat. no. 18505‐1‐AP; Proteintech Group, Inc.) and PDGF‐D (1:100 dilution; cat. no. 14075‐1‐AP; Proteintech Group, inc.). afterward, the sections were stained for 1 h with the following secondary antibodies: Goat anti‐mouse IgG H&L (1:500; cat. no. ab150113; Abcam) and goat anti‐rabbit IgG H&L (1:500; cat. no. ab150080; Abcam). .. Finally, cell nuclei in the tissue sections were labeled using 4',6‐diamidino‐2‐phe‐ nylindole (cat. no. F6057; Sigma‐Aldrich; Merck KGaA).

    Produced:

    Article Title: Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.
    Article Snippet: .. Then, the sections were incubated with monoclonal anti-PDGF-A, PDGF-B, PDGF-C (Santa Cruz Biotechnology, Inc, USA), and PDGF-D (Proteintech Group, Inc, USA) antibodies and antiPDGFR-a and PDGFR-b (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies produced in rabbit (1:50) for 18 h at 4 C. Subsequently, the sections were incubated with a secondary biotinylated anti-rabbit IgG antibody (Santa Cruz Biotechnology), diluted 1:200 in phosphate-buffered saline containing 1% normal goat serum for 45 min. .. The sections were incubated with avidin-biotin-HRP complex (1:600, Vectastain Elite ABC kits; Vector Laboratories, Burlingame, CA, USA) for 45 min.

    Saline:

    Article Title: Differential gene expression and immunolocalization of platelet-derived growth factors and their receptors in caprine ovaries.
    Article Snippet: .. Then, the sections were incubated with monoclonal anti-PDGF-A, PDGF-B, PDGF-C (Santa Cruz Biotechnology, Inc, USA), and PDGF-D (Proteintech Group, Inc, USA) antibodies and antiPDGFR-a and PDGFR-b (Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies produced in rabbit (1:50) for 18 h at 4 C. Subsequently, the sections were incubated with a secondary biotinylated anti-rabbit IgG antibody (Santa Cruz Biotechnology), diluted 1:200 in phosphate-buffered saline containing 1% normal goat serum for 45 min. .. The sections were incubated with avidin-biotin-HRP complex (1:600, Vectastain Elite ABC kits; Vector Laboratories, Burlingame, CA, USA) for 45 min.



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